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GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis |
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| Authors: | Simon Stael Liana Tsiatsiani Hanna Help‐Rinta‐Rahko Adrien Gauthier David Kaufholdt Benjamin Bollhöner Airi Lamminmäki An Staes Kris Gevaert Hannele Tuominen Frank Van Breusegem Ykä Helariutta Jaakko Kangasjärvi |
| Affiliation: | 1. Department of Plant Systems Biology, VIB, Ghent, Belgium;2. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium;3. Department of Medical Protein Research, VIB, Ghent, Belgium;4. Department of Biochemistry, Ghent University, Ghent, Belgium;5. Plant Biology, Department of Biosciences, University of Helsinki, Helsinki, Finland;6. Institute of Biotechnology, University of Helsinki, Helsinki, Finland;7. Ume? Plant Science Centre, Department of Plant Physiology, Ume? University, Ume?, Sweden;8. The Sainsbury Laboratory, University of Cambridge, Cambridge, UK;9. Distinguished Scientist Fellowship Program, College of Science, King Saud University, Riyadh, Saudi Arabia |
| Abstract: | Recognition of extracellular peptides by plasma membrane-localized receptor proteins is commonly used in signal transduction. In plants, very little is known about how extracellular peptides are processed and activated in order to allow recognition by receptors. Here, we show that induction of cell death in planta by a secreted plant protein GRIM REAPER (GRI) is dependent on the activity of the type II metacaspase METACASPASE-9. GRI is cleaved by METACASPASE-9 in vitro resulting in the release of an 11 amino acid peptide. This peptide bound in vivo to the extracellular domain of the plasma membrane-localized, atypical leucine-rich repeat receptor-like kinase POLLEN-SPECIFIC RECEPTOR-LIKE KINASE 5 (PRK5) and was sufficient to induce oxidative stress/ROS-dependent cell death. This shows a signaling pathway in plants from processing and activation of an extracellular protein to recognition by its receptor. |
| Keywords: | ligand protease receptor-like kinase secreted protein |