| 简并PCR技术及其在基因克隆中的应用 |
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| 引用本文： | 王洪振,周晓馥,宋朝霞,刘文广,曾宪录. 简并PCR技术及其在基因克隆中的应用[J]. 遗传, 2003, 25(2): 201-204 |
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| 作者姓名： | 王洪振 周晓馥 宋朝霞 刘文广 曾宪录 |
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| 作者单位： | 1.东北师范大学遗传与细胞研究所，长春 130024；2.吉林师范大学生物系，四平 1360001.Institute of Genetics and Cell Biology,Northeast Normal University,Changchun 130024,China;2.Department of Biology,Jilin Normal University,Siping 136000,China |
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| 基金项目： | 国家自然科学基金(批准号:30040031)，吉林省自然科学基金(批准号:20010553)资助项目 |
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| 摘 要： | 本文简要介绍简并PCR技术，包括什么是简并引物，如何设计简并引物，进行简并PCR的反应条件，应用简并PCR获得全长基因的方法和简并PCR技术的应用范围，并对简并PCR技术的局限性及其新进展进行讨论。在此基础上，简述基因的克隆策略以及简并PCR技术在基因克隆中的应用。简并PCR技术是寻找和发现“新”基因或蛋白质家族新成员的一种非常有用的工具。Abstract:Degenerate PCR is introduced in this paper,including what is degenerate PCR,how to design degenerate primers,how to optimize degenerate PCR parameters,how to applying degenerate PCR to obtain full-length gene and which fields can apply degenerate PCR.The limits and recent advances of degenerate PCR are also discussed.Based on this introduction,strategies of gene cloning and applications of degenerate PCR in gene cloning are summarized in brief.Degenerate PCR is a very useful tool for searching and discovering new genes and new members of a protein family.
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| 关 键 词： | degenerate PCR degenerate primer 基因克隆 Key words 简并引物 简并PCR |
| 文章编号： | 0253-9772(2003)02-0201-04 |
| 修稿时间： | 2002-04-29 |
Degenerate PCR and Its Application in Gene Cloning |
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| Hong-Zhen Wang,Xiao-Fu Zhou,Zhao-Xia Song,Wen-Guang Liu,Xian-Lu Zeng. Degenerate PCR and Its Application in Gene Cloning[J]. Hereditas, 2003, 25(2): 201-204 |
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| Authors: | Hong-Zhen Wang Xiao-Fu Zhou Zhao-Xia Song Wen-Guang Liu Xian-Lu Zeng |
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| Affiliation: | Institute of Genetics and Cell Biology, Northeast Normal University, Changchun 130024,China. biostudent@sohu.com |
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| Abstract: | Degenerate PCR is introduced in this paper, including what is degenerate PCR, how to design degenerate primers, how to optimize degenerate PCR parameters, how to applying degenerate PCR to obtain full-length gene and which fields can apply degenerate PCR. The limits and recent advances of degenerate PCR are also discussed. Based on this introduction,strategies of gene cloning and applications of degenerate PCR in gene cloning are summarized in brief. Degenerate PCR is a very useful tool for searching and discovering new genes and new members of a protein family. |
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| Keywords: | degenerate PCR degenerate primer gene cloning |
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