Characterization of hypoxanthine-guanine phosphoribosyl transferase in man-mouse somatic cell hybrids by an improved electrophoretic method |
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| Authors: | Seung-il Shin P. Meera Khan Peter R. Cook |
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| Affiliation: | (1) Department of Human Genetics, University of Leiden, The Netherlands;(2) Stichting voor Fundamenteel Geneeskundig Onderzoek (FUNGO) of the Dutch Organization for Pure Scientific Research (ZWO), The Netherlands;(3) Present address: Basel Institute for Immunology, Basel, Switzerland;(4) Sir William Dunn School of Pathology, University of Oxford, England;(5) Brasenose College, Oxford |
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| Abstract: | The hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity in a group of man-mouse somatic cell hybrids, produced by Sendai virus-mediated cell fusion and HAT selection, has been analyzed by a new electrophoretic technique. Evidence is presented which shows that the hybrid lines derived from fusion of a mouse fibroblast deficient in HGPRT with various human cell strains have an HGPRT activity that is characteristic of the human enzyme, whereas a hybrid line derived from a mouse fibroblast which is deficient in thymidine kinase has an HGPRT activity characteristic of the mouse. This new technique involves electrophoresis of cell extracts on cellulose acetate gel, followed by the localization of the enzyme activity by autoradiography.This research was supported in part by a research grant from the U.S. National Institutes of Health (No. GM-13415). |
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