Isolation from cattle of a prion strain distinct from that causing bovine spongiform encephalopathy |
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Authors: | Béringue Vincent Bencsik Anna Le Dur Annick Reine Fabienne Laï Thanh Lan Chenais Nathalie Tilly Gaëlle Biacabé Anne-Gaëlle Baron Thierry Vilotte Jean-Luc Laude Hubert |
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Institution: | Institut National de la Recherche Agronomique, Virologie Immunologie Moléculaires, Jouy-en-Josas, France. |
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Abstract: | To date, bovine spongiform encephalopathy (BSE) and its human counterpart, variant Creutzfeldt-Jakob disease, have been associated with a single prion strain. This strain is characterised by a unique and remarkably stable biochemical profile of abnormal protease-resistant prion protein (PrP(res)) isolated from brains of affected animals or humans. However, alternate PrP(res) signatures in cattle have recently been discovered through large-scale screening. To test whether these also represent separate prion strains, we inoculated French cattle isolates characterised by a PrP(res) of higher apparent molecular mass--called H-type--into transgenic mice expressing bovine or ovine PrP. All mice developed neurological symptoms and succumbed to these isolates, showing that these represent a novel strain of infectious prions. Importantly, this agent exhibited strain-specific features clearly distinct from that of BSE agent inoculated to the same mice, which were retained on further passage. Moreover, it also differed from all sheep scrapie isolates passaged so far in ovine PrP-expressing mice. Our findings therefore raise the possibility that either various prion strains may exist in cattle, or that the BSE agent has undergone divergent evolution in some animals. |
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