Methionine-defective mutants inCandida utilis

Ethionine-resistant mutants ofCandida utilis CCY-158 overproducing methionine have been isolated. In these mutants the intracellular methionine concentration decreased significantly during the stationary phase. The wild-type strain CCY-158 and the ethionine-resistant mutants isolated were able to use methionine as the nitrogen source but not as the carbon source. From these ethionine-resistant mutants we isolated mutants unable to use methionine as nitrogen source (Mec^- mutants), the principal alteration being at the level of methionine uptake. Some of the Mec mutants lost also the ability to use other amino acids as nitrogen source.