Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other gram negative bacteria |
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Authors: | Graham J. Boulnois Jennifer M. Varley Geoffrey S. Sharpe F. Christopher H. Franklin |
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Affiliation: | (1) Department of Medical Biochemistry, University Medical Centre, 9, Avenue de Champel, CH-1211 Geneva 4, Switzerland;(2) Present address: University/ICI Joint Laboratory, University of Leicester, University Road, LE1 7RH Leicester, UK;(3) Present address: Department of Microbiology, University of Leicester, University Road, LE1 7RH Leicester, UK;(4) Present address: Department of Genetics, University of Birmingham, Birmingham, UK |
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Abstract: | Summary The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria. |
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