Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other gram negative bacteria |
| |
| Authors: | Graham J Boulnois Jennifer M Varley Geoffrey S Sharpe and F Christopher H Franklin |
| |
| Institution: | (1) Department of Medical Biochemistry, University Medical Centre, 9, Avenue de Champel, CH-1211 Geneva 4, Switzerland;(2) Present address: University/ICI Joint Laboratory, University of Leicester, University Road, LE1 7RH Leicester, UK;(3) Present address: Department of Microbiology, University of Leicester, University Road, LE1 7RH Leicester, UK;(4) Present address: Department of Genetics, University of Birmingham, Birmingham, UK |
| |
| Abstract: | Summary The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
