红豆杉细胞时相与其次生代谢强度的关系

研究了处于不同时相的红豆杉细胞经诱导后生活力、生物量、紫杉醇含量及几个与次生代谢有关的生理化指标的差异，对细胞时相与次生代谢强度的关系进行了探讨。结果表明，在细胞培养的第７ｄ（延迟期）进行诱导，细胞的活力、ＰＯＤ活性、Ｈ２Ｏ２、生物量、紫杉醇含量均高于第１４ｄ（对数期）进行诱导，显著高于第２１ｄ（稳定期）开始诱导。说明在第７ｄ（延迟期）进行诱导时，细胞对诱导子的反应灵敏度较高，次生代谢启动的强度更

Relationships between the strength of secondary metabolite of Taxus cell and cell phase in suspension culture system

The changes of the life ability,biomass,taxol content,POD activity and H 2O 2 content of Taxus cells were studied when thdy were induced at different cell phase.The results indicated that when the cells in lag phase were induced,the life ability,biomass,taxol content,POD activity and H 2O 2 content increased more than that of cells which were induced in exponential phase and markedly higher that of cells which were induced in stationary phase.