| 大豆全基因组分枝相关基因发掘及与QTL共定位 |
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| 引用本文： | 谭冰,郭勇,邱丽娟. 大豆全基因组分枝相关基因发掘及与QTL共定位[J]. 遗传, 2013, 35(6): 793-804. DOI: 10.3724/SP.J.1005.2013.00793 |
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| 作者姓名： | 谭冰 郭勇 邱丽娟 |
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| 作者单位： | 1. 东北农业大学大豆研究所, 哈尔滨150030 2. 中国农业科学院作物科学研究所, 国家农作物基因资源与遗传改良重大科学工程, 北京100081 |
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| 基金项目： | 国家高技术研究发展计划(863,计划)项目(项目编号：2012AA101106),国家自然科学基金项目(项目编号：30490251) |
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| 摘 要： | 大豆(Glycine max)分枝在个体和群体水平上均与大豆产量关系密切, 因此大豆分枝相关基因的发掘及利用对大豆高产分子育种具有重要意义。文章通过GO(Gene ontology)分类和文献检索共获得植物分枝发育相关基因183个。基于序列相似和结构域相同的原则, 从大豆基因组中发掘出大豆分枝相关的候选基因406个。通过收集已发表的大豆分枝相关QTL, 利用BioMercator2.1软件, 将符合映射条件的35个QTL映射到公共图谱的12个染色体。通过共定位分析发现, 在20个分枝相关的QTL区间内存在大豆分枝相关候选基因57个。本文发掘的分枝发育相关基因信息为大豆分枝相关QTL的精细定位和克隆以及大豆分枝发育的分子生物学基础研究提供了参考。
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| 关 键 词： | 共定位 大豆 分枝 基因 QTL |
| 收稿时间： | 2012-11-14 |
Whole genome discovery of genes related to branching and co-localization with QTLs in soybean |
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| Affiliation: | 1. Soybean Research Institute, Northeast Agricultural University, Harbin 150030, China 2. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Branch number of soybean is one of agronomic traits that closely related with yield in both individual and population levels; therefore, discovering genes related to branching is important for molecular breeding in soybean. In this study, 183 genes related to plant branch development had been collected through GO classification and literatures. Based on the principle of similar sequences and conserved domains, 406 genes possibly related to branching had been identified at the whole genome level in soybean. On the other hand, integration of published QTLs related to branching was carried out using BioMercator2.1, and a total of 35 QTLs was located on 12 chromosomes. Co-localization analysis of QTLs and genes related to branching suggested that 57 genes were located in 20 intergrated QTL regions. These results provided useful in-formation for fine mapping of QTLs related to branching and molecular biology study of branch development in soybean. |
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| Keywords: | soybean branches genes QTL co-localization |
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