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阳桃的组织培养与快速繁殖
引用本文:刘建福,吴清.阳桃的组织培养与快速繁殖[J].热带亚热带植物学报,2004,12(3):265-267.
作者姓名:刘建福  吴清
作者单位:1. 华侨大学生物工程系,福建,泉州,362021
2. 四川省宜宾市园林管理局,四川,宜宾,644000
基金项目:华侨大学科研基金项目(03HZR1)资助
摘    要:以阳桃茎段为材料进行组培快繁。结果表明:阳桃离体培养的细胞分裂素以ZT的效果最好,其次是BA,KT最差;植物生长调节物质是NAA最好,其次是IBA、IAA;较适宜的植物生长调节物质配比为BA 0.5mg L^-1 NAA 0.2mg L^-1 GA 0.2mg L^-1,增殖系数高达3.7,而且畸苗率较低(23.3%)。生根培养基以1/2MS IBA 0.2mg L^-1 IAA 0.1mg L^-1效果好,生根率达87.1%,蔗糖浓度以2.0%一3.0%为宜。

关 键 词:阳桃  离体培养  快繁  茎段
文章编号:1005-3395(2004)03-0265-03
修稿时间:2003年7月16日

Rapid in vitro Propagation of Averrhoa carambola
LIU Jian-fu,WU Qing.Rapid in vitro Propagation of Averrhoa carambola[J].Journal of Tropical and Subtropical Botany,2004,12(3):265-267.
Authors:LIU Jian-fu  WU Qing
Abstract:Nodal regments of tropical fruit Averrhoa carambola (Oxalidaceae) were used as explants to investigate optimum conditions for rapid propagation. Among cytokinins (1.0 mg L-1), zeatin (1.0 mg L-1) was best for culture, and kinetin (1.0 mg L-1) the worst. MS medium supplemented with 0.5 mg L-1 BA, 0.2 mg L-1 NAA and 0.2 mg L-1 GA was optimal for the plantlet growth, the proliferation coefficient being as high as 3.7, and the rate of abnormal plantlets was only 23.3%. As for rooting, half-strengh MS medium containing 0.2 mg L-1 IBA, 0.1 mg L-1 IAA and 2%-3% sucrose was the best. The rooting rate was 87.1%.
Keywords:Averrhoa carambola  Tissue culture  Rapid propagation  Stem
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