| 田埂报春多倍体诱导及其形态学研究 |
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| 引用本文： | 张晓曼,孙晓光,杨建民.田埂报春多倍体诱导及其形态学研究[J].植物遗传资源学报,2010,11(6):789-792. |
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| 作者姓名： | 张晓曼 孙晓光 杨建民 |
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| 作者单位： | 1. 河北农业大学园林与旅游学院,保定,071000
2. 保定市交通局公路管理处,保定,071000 |
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| 基金项目： | 河北省科技支撑计划项目 |
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| 摘 要： | 在离体培养条件下,比较不同浓度、不同处理时间的秋水仙素对田埂报春进行染色体加倍的诱导效果。结果表明:0.08%秋水仙素处理48h的诱变效果最佳,诱变率高达56%。经秋水仙素诱导后形成的多倍体植株与原二倍体植株比较,在形态上,四倍体植株表现出多倍体特征,叶片变厚,叶形指数减小,保卫细胞增大,单位面积气孔数减少,叶绿体数明显增多。对变异植株进行细胞学研究发现,体细胞中期染色体数目为2n=4x=36,而原二倍体的染色体数目为2n=2x=18,基数x=9,因此,变异植株(2n=4x=36)为四倍体。前者的核型公式为2n=4x=8L+12M2+4M1+12S,核型属于1A;后者的核型公式为2n=2x=4L+6M2+2M1+6S,核型也属于1A。检测发现少数个体有非整倍体变异。
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| 关 键 词： | 田埂报春 秋水仙素 四倍体 染色体 核型 |
Polyploid Induction and Morphology of Primula forbesii |
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| ZHANG Xiao-man,SUN Xiao-guang,YANG Jian-min.Polyploid Induction and Morphology of Primula forbesii[J].Journal of Plant Genetic Resources,2010,11(6):789-792. |
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| Authors: | ZHANG Xiao-man SUN Xiao-guang YANG Jian-min |
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| Institution: | ZHANG Xiao-man1,SUN Xiao-guang2,YANG Jian-min1(1College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding 071000,2Highway Administrative Office of Transportation Bureau of Baoding,Baoding 071000) |
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| Abstract: | In vitro culture of Primula forbesii was treated with colchicine at different concentrations and durations.The results showed that the induction rate was 56% with 0.08% colchicine for 48h.Compared with the normal diploid plants,the polyploid plants showed the general characteristics of tetraploids,such as thicker leaf,smaller leaf index,larger guard cells,less stomata perunit area and more chloroplasts in guard cells.Cytological observation showed the chromosome number of mutant plants was 2n=4x=36,while th... |
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| Keywords: | Primula forbesii Colchicine Tetraploid Chromosome Karyotype |
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