Stimulation of the methyltetrahydromethanopterin: coenzyme M methyltransferase reaction in cell-free extracts of Methanobacterium thermoautotrophicum by the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate |
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Authors: | Servé W M Kengen Piet J H Daas Jan T Keltjens Chris van der Drift Godfried D Vogels |
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Institution: | (1) Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands |
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Abstract: | The conversion of formaldehyde to methylcoenzyme M in cell-free extracts of Methanobacterium thermoautotrophicum was stimulated up to 10-fold by catalytic amounts of the heterodisulfide (CoM-S-S-HTP) of coenzyme M and 7-mercaptoheptanoylthreonine phosphate. The stimulation required the additional presence of ATP, also in catalytic concentrations. ATP and CoM-S-S-HTP were mutually stimulatory on the methylcoenzyme M formation and it was concluded that the compounds were both involved in the reductive activation of the methyltetrahydromethanopterin: coenzyme M methyltransferase. Micromolar concentrations of benzyl viologen or cyanocobalamin inhibited the formaldehyde conversion; these compounds, however, strongly stimulated the reduction of CoM-S-S-HTP. The results described here closely resemble observations made on the activation and reduction of CO2 to formylmethanofuran indicating that this step and the reductive activation of the methyltransferase are controlled by some common mechanism.Abbreviations HS-CoM
Coenzyme M, 2-mercaptoethanesulfonate
- CH3S-CoM
methylcoenzyme M, 2-(methylthio)ethanesulfonate
- H4MPT
5,6,7,8-tetrahydromethanopterin
- MFR
methanofuran
- HS-HTP
7-mercaptoheptanoylthreonine phosphate
- CoM-S-S-HTP
the heterodisulfide of HS-CoM and HS-HTP
- BES
2-bromoethanesulfonate
- TES
N-tris(hydroxymethyl)methyl-2-aminoethanesulfonate
- CN-Cbl
cyanocobalamin
- HO-Cbl
hydroxycobalamin
- HBI
5-hydroxybenzimidazole
- DMBI
5,6-dimethylbenzimidazole |
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Keywords: | Methanobacterium thermoautotrophicum Coenzyme M 7-Mercaptoheptanoylthreonine phosphate 5-Methyltetrahydromethanopterin: coenzyme M methyltransferase Corrinoid enzyme Reductive activation |
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