Membrane adhesion in photosynthetic bacterial membranes. Light harvesting complex I (LHI) appears to be the main adhesion factor |
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Authors: | Amy R. Varga L. Andrew Staehelin |
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Affiliation: | (1) Department of Molecular, Cellular and Developmental Biology, University of Colorado, 80309 Boulder, CO, USA;(2) Present address: Department of Microbiology, University of Illinois, 61801 Urbana, IL, USA |
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Abstract: | We have reconstituted pigment-protein complexes isolated from Rhodopseudomonas palustris photosynthetic membranes into phospholipid liposomes. The various complexes were tested for their ability to promote adhesion of the liposome membrane in the presence and absence of Mg2+ ions. Samples containing a reaction center (RC)/light-harvesting I (LHI) complex appeared to stack in a manner resembling control thylakoids in 2 and 5 mM Mg2+. We also tested for the effects of Mg2+ on detergent extractablity of pigment-protein complexes from intact membranes. Mg2+ sharply reduced the amount of LHI solubilized from membranes, while having little effect on the extractability of the light harvesting II complex (LHII) and the RC. Based on these results we suggest that LHI is the principal adhesion factor of R. palustris thylakoids.Abbreviations LHC light harvesting complex - OG octyl glucoside - RC reaction centerThis paper is dedicated to Professor G. Drews on the occasion of his 60th birthday |
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Keywords: | Membrane adhesion Reconstitution Liposomes Pigment-proteins Freeze-fracture Rhodopseudomonas palustris |
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