Observations on enzymatically isolated,living and fixed embryo sacs in several angiosperm species |
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Authors: | C Zhou H Y Yang |
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Institution: | (1) Department of Biology, Wuhan University, Wuhan, China |
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Abstract: | A technique has been developed for isolating embryo sacs (ESs) by enzymatic maceration. Ovules were macerated in a mixture of pectinase, cellulase and, in some cases, snailase and pectolyase Y-23. The ovular tissues were removed and the ESs were isolated in toto. Embryo sacs were isolated from both fixed and fresh ovules of Antirrhinum majus L., Helianthus annuus L. and Nicotiana tabacum L. Fluorochromasia by fluorescein diacetate showed that the ESs isolated from fresh ovules were viable. The method has promise for various histochemical and cell-physiological studies and quite possibly also for in-vitro culture of ESs.Abbreviations ES
embryo sac
- FDA
fluorescein diacetate
- FPA
formalin-propionic acid 50% alcohol (5:5:10, by vol.)
- H33258
Hoechst 33258 |
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Keywords: | Antirrhinum (embryo-sac isolation) Embryo sac (isolated) Helianthus (embryo-sac isolation) Nicotiana (embryo-sac isolation) |
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