PREPARATION OF SUCCINIC SEMIALDEHYDE: ITS COLORIMETRIC ESTIMATION AS AN ASSAY FOR GABA AMINOTRANSFERASE |
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| Authors: | P V Taberner J E G Barnett G A Kerkut |
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| Institution: | Department of Physiology and Biochemistry, Southampton University |
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| Abstract: | Abstract— A simple method is described for the preparation of succinic semialdehyde by the oxidation of 4-hydroxybutyric acid with dimethyl sulphoxide in the presence of phosphorus pentoxide. The colorimetric estimation of succinic semialdehyde by condensation with 3-methyl-2-benzothiazolinone-2-hydrazone is used as an assay for GABA aminotransferase activity in mouse brain. The values obtained for the specific activity of this enzyme in mouse brain using the colorimetric method agree with those obtained by the more conventional isotopic method. |
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