Thrombospondin production and thrombospondin-mediated adhesion in U937 cells |
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Authors: | J Varani L Stoolman T Wang L Schuger C Flippen M Dame K J Johnson R F Todd U S Ryan P A Ward |
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Affiliation: | Department of Pathology, University of Michigan Medical School, Ann Arbor 48109. |
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Abstract: | U937 cells have low levels of surface thrombospondin (TSP) under control conditions but express higher levels after treatment for 1 day with 100 nM phorbol myristate acetate (PMA). Increased surface expression is due, in part, to increased biosynthesis. Untreated U937 cells do not adhere to TSP-coated plastic culture dishes but adhere strongly to TSP after stimulation with PMA. Untreated U937 cells also adhere weakly to endothelial cell monolayers while PMA-treated U937 cells attach strongly to monolayers of rat pulmonary artery endothelial cells. Endothelial cell adhesion appears to be mediated, in part, by TSP since antibodies to TSP partially inhibit. |
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