Purification and characterization of a new peptide antibiotic produced by a thermotolerant Bacillus licheniformis strain |
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Authors: | Sónia Mendo Nuno André Faustino Ana Cristina Sarmento Francisco Amado Arthur J.G. Moir |
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Affiliation: | Departamento de Biologia, Centro de Biologia Celular, Universidade de Aveiro, 3810-193 Aveiro, Portugal. smendo@bio.ua.pt |
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Abstract: | A Bacillus licheniformis strain, 189, isolated from a hot spring environment in the Azores, Portugal, strongly inhibited growth of Gram-positive bacteria. It produced a peptide antibiotic at 50 degrees C. The antibiotic was purified and biochemically characterized. It was highly resistant to several proteolytic enzymes. Additionally, it retained its antimicrobial activity after incubation at pH values between 3.5 and 8; it was thermostable, retaining about 85% and 20% of its activity after 6 h at 50 degrees C and 100 degrees C, respectively. Its molecular mass determined by mass spectrometry was 3249.7 Da. |
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Keywords: | peptide antibiotics peptide synthetases thermotolerant Bacillus |
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