Isolation of Serratia marcescens mutants which could overproduce and excrete Escherichia coli alkaline phosphatase and beta-lactamase |
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Authors: | S Asdornnithee K Akiyama R Takata |
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Institution: | (1) Department of Biotechnology, College of Agriculture, Ehime University, 3-5-7 Tarumi, 790 Matsuyama, Ehime, Japan |
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Abstract: | Serratia marcescens mutants, which excrete Escherichia coli alkaline phosphatase (APase) encoded by the plasmid-bearing phoA gene, were isolated after mutagenesis by N-methyl- -nitro-N-nitrosoguanidine. These mutants produced two to four times as much APase as did the parent strain under a phosphate-limiting condition, and more than 70% of the enzyme was released into the culture medium. In addition, overproduction and excretion of beta-lactamase was observed in these mutants. |
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Keywords: | Serratia marcescens APase Overproduction Beta-lactamase excretion |
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