Blocking BE301622 gene expression by RNAi initiates differentiation of neural stem cells in rat |
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Authors: | Wen Tieqiao Bao Kuiyi Li Hailong |
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Institution: | Laboratory of Neural Molecular Biology, School of Life Sciences, Shanghai University, Shanghai, China. tqwen@mail.shu.edu.cn |
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Abstract: | Neural stem cells (NSCs) are capable of differentiating into neurons, astrocytes and oligodendrocytes. However, the molecular mechanisms regulating NSCs differentiation are not well understood. Our previous research by microarray analysis certified that a lot of genes are differentially expressed in the course of NSC differentiation. In this study we report the function of one of these genes, BE301622, by RNAi techniques. To silence the BE301622 gene, a long, double-stranded RNA (dsRNA) was synthesized by using a kit (Ambion T7 MegaScript) and transformed into NSCs. Expression of mRNA was tested through RT-PCR. The result showed the expression of BE301622 gene was specificially suppressed. This finding effectively validated that BE301622 is involved in the differentiation of NSCs. |
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Keywords: | neural stem cells differentiation RNAi dsRNA RT‐PCR |
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