Chemotactic factor causes rapid decreases in phosphatidylinositol,4,5-bisphosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophils |
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| Authors: | M Volpi R Yassin P H Naccache R I Sha'afi |
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| Institution: | 1. Department of Physiology, University of Connecticut Health Center, Farmington, CT. 06032 USA;2. Department of Pathology, University of Connecticut Health Center, Farmington, CT. 06032 USA |
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| Abstract: | Stimulation of rabbit neutrophils prelabeled with 32P by the synthetic chemotactic peptide f-Met-Leu-Phe induces a rapid decrease in the radioactivity in both phosphatidylinositol, 4,5 bis phosphate and phosphatidylinositol 4-monophosphate. The mean +/- standard error of the mean values of the maximum decrease in phosphatidylinositol, 4,5 bis phosphate occurred at 10 seconds following stimulation and is equal to 19 +/- 3% of the control value. The corresponding value for phosphatidylinositol 4-monophosphate occurred at 60 seconds following stimulation and is equal to 37 +/- 7% of the control value. On the other hand, the radioactivity in phosphatidic acid and lysophospholipids increased continuously with time following stimulation. The relationship of these changes to calcium release and neutrophil activation is discussed. |
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| Keywords: | TPI phosphatidylinositol 4 5-bis phosphate DPI phosphatidylinositol 4-monophosphate LPI lysophosphatidylinositol PA phosphatidic acid PI phosphatidylinositol PC phosphatidylcholine LP lysophospholipid PE phosphatidylethanolamine TLC thin layer chromatography EGTA ethyleneglycol-bis-(β-amino-ethyl-ether)N N′-tetra-acetic acid |
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