Improved production of biosurfactant by a Pseudomonas aeruginosamutant using vegetable oil refinery wastes |
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| Authors: | Zulfiqar Ali Raza Asma Rehman Muhammad Saleem Khan Zafar M. Khalid |
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| Affiliation: | (1) Polymer Chemistry Laboratory, National Centre of Excellence in Physical Chemistry, University of Peshawar, 25210 Peshawar, Pakistan;(2) Environmental Biotechnology Division, National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan |
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| Abstract: | Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l−1) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l−1) and rhamnolipid production (8.50 g l−1), at 10 d of incubation with the specific growth rate of 0.316 h−1 and specific product yield of 0.597 g g−1 h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m−1. |
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| Keywords: | biosurfactant emulsion Pseudomonas aeruginosa vegetable oil wastes yield factors |
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