Kinetic analysis of oil biosynthesis by an arachidonic acid-producing fungus, Mortierella alpina 1S-4 |
| |
Authors: | M Certik S Shimizu |
| |
Institution: | (1) Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan e-mail: sim@kais.kyoto-u.ac.jp Tel.: +81-75-7536115 Fax: +81-75-7536128, JP |
| |
Abstract: | Kinetic analysis of arachidonic acid (AA)-oil biosynthesis by Mortierella alpina 1S-4 growing under lipid-accumulating (LN medium) and non-lipid-accumulating (HN medium) conditions was investigated and
compared with industrial AA fermentation. Various kinetic parameters of these cultivation processes demonstrate a characteristic
pattern of the lipogenesis in this fungus, where growth phase, phase of oil accumulation and phase of AA synthesis are distinct
from each other. The fungus utilizing LN medium synthesized 32.3 g fatty acid 100 g−1 glucose on the 4th day of cultivation and reached the maximum daily fatty acid accumulation (expressed as differential specific
rate q
D(FA/B)) of 9.5%. Our results also indicate that a q
D(FA/B) value of about 2.5% might be critical for lipid overproduction in M. alpina. AA was rapidly incorporated into triacylglycerols (90% of total AA) at the later cultivation phase and overall AA yield was
directly related to the total yield of fatty acid.
Received: 10 December 1999 / Accepted: 13 February 2000 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|