Characterization of lactoferrin binding to the MAC-T bovine mammary epithelial cell line using a biotin-avidin technique |
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Institution: | 1. Department of Pharmacology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey;2. Center for Nanotechnology and Biomaterials Application and Research, Marmara University, Istanbul, Turkey |
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Abstract: | - 1.1. A non-radioisotopic method utilizing a biotin-avidin approach was used to characterize lactoferrin binding to the clonal MAC-T bovine mammary epithelial cell line.
- 2.2. Binding of lactoferrin to MAC-T cells and isolated membranes was specific and saturable.
- 3.3. Unlabeled lactoferrin competed for and displaced biotin-labeled lactoferrin from binding sites on mammary epithelial cells. In contrast, unlabeled transferrin did not compete.
- 4.4. Scatchard analysis of lactoferrin binding to MAC-T cell crude membranes was nonlinear, revealing two classes of binding sites with association constants (Ka) of 2.36 × 107 and 3.36 × 106M?1.
- 5.5. Binding of lactoferrin to MAC-T cells may be associated with the initial events which result in decreased MAC-T cell proliferation.
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