Juvenile hormone counteracts the action of ecdysterone on silk glands of Galleria mellonella L. (Lepidoptera : Pyralidae) |
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| Affiliation: | 1. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Chiyoda-Ward, Tokyo 101-8308, Japan;2. Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Ohtsu, Shiga 520-2192, Japan;3. Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan;1. Department of Pharmacology and New Drug Development Institute, Medical School, Chonbuk National University, Jeonju, Chonbuk 561-756, Republic of Korea;2. Clinical Trial Center for Functional Foods (CTCF2), Chonbuk National University Hospital, Jeonju, Chonbuk 561-712, Republic of Korea;3. Functional Food Center at Chonbuk National University, Jeonju, Chonbuk, 561-756, Republic of Korea;4. Buan Agricultural Development & Technology Center, Buan-gun, Jeonbuk, 579-833, Republic of Korea;1. Department of Science, University of Basilicata, Viale dell’Ateneo Lucano, 10, 85100, Potenza, Italy;2. School of Agricultural Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Viale dell’Ateneo Lucano, 10, 85100, Potenza, Italy;3. Bioinnova srls, Via ponte nove luci, 22, 85100, Potenza, Italy;1. Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, Siedlce University of Natural Sciences and Humanities, 3 Maja 54, 08-110 Siedlce, Poland;2. Section of Toxicology and Bioanalytics, Faculty of Civil Engineering, Environmental and Geodetic Sciences, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland |
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| Abstract: | Morphological and physiological evidence is presented to show that ecdysterone (20E) and juvenile hormone (JH) directly affect the silk glands of Galleria mellonella (Lepidoptera : Pyralidae). Within 1 hr in a culture medium, 20E at 5 or 50 ng/ml stimulates, and at 5 μg/ml inhibits, RNA synthesis. Both these effects are obliterated with physiological (1 ng/ml) and higher doses of JH II or a juvenoid. Dipping of isolated larval abdomens in 0.32% 20E suppresses the rate of RNA synthesis in freshly dissected silk glands incubated in a hormone-free medium. The ultrastructure of silk glands shows functional regression, followed by histolysis within 72 hr after dipping. Both the reduction of RNA synthesis and the cytological changes are prevented when the abdomens receive JH II or a juvenoid simultaneously with 20E. Presence of JH II in the culture medium also enhances RNA synthesis in silk glands dissected from abdomens that had been treated with 20E. The results reveal that the effect of 20E is dose-dependent and may be prevented, and up to a certain point reversed, with JH. |
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