Hemolysis and clearance of erythrocytes in Scylla serrata are related to the agglutination by the native sialic acid-specific lectin |
| |
| Affiliation: | 1. Department of Zoology, Holy Cross College, Roche Nagar, Nagarcoil, Tamilnadu, India 629004;2. Laboratory of Glycolipid Immunotherapy, John Wayne Cancer Institute, 2200 Santa Monica Blvd, Santa Monica, CA 90404, U.S.A.;1. Biochemistry Department, Center for Pharmaceuticals Research and Development, Havana, Cuba;2. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico;3. Department of Fish Physiology and Biotechnology, Institute of Aquaculture Torre de la Sal (IATS-CSIC), Castellón, Spain;4. Center for Marine Research, University of Havana, Havana, Cuba;5. Department for Basic and Biomedical Sciences, Medicine Faculty, Artemisa, Cuba;6. Laboratory of Computational and Theoretical Chemistry, University of Havana, Havana, Cuba;7. Center for Protein Studies, Faculty of Biology, University of Havana, Havana, Cuba;8. Toxicology and Pharmacology, University of Leuven (KU Leuven), Leuven, Belgium;1. CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China;3. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China;4. University of Chinese Academy of Sciences, Beijing, 100049, China;5. School of Marine Science, Ningbo University, Zhejiang, Ningbo, 315211, China;1. School of Chemistry, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India;2. Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India;1. Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block 4th Floor, Burma Colony, Karaikudi, 630004, Tamil Nadu, India;2. School of Bioscience & Technology, VIT University, Vellore, 632014, Tamil Nadu, India |
| |
| Abstract: | The hemolymph of the crab Scylla serrata contains a lectin specific for N-glycolylneuraminic acid. The role of the sialic acid-specific lectin on natural immunity of the crab is studied by using several kinds of mammalian erythrocytes as a pathogen model. A significant correlation is observed between in vivo clearance of exogenous erythrocytes with the extent of erythrocyte agglutination by the lectin. Similarly, another correlation is noticed between the susceptibility of erythrocytes to lectin-dependent hemocytc-mediated hemolysis and the extent of lectin-mediated erythrocyte agglutination. Two hours after administration of the erythrocytes into the hemocoel, induced augmentation of hemagglutinating activity was observed against all erythrocytes, whether agglutinated highly or least by the lectin, suggesting an increase in the circulating lectin. This study documents that “opsonization” of foreign pathogen by the native lectin is an important step in hemocyte recognition, hemolysis and clearance of the pathogen. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
