Eicosapentaenoic acid enhances myo-inositol uptake in cultured human aortic smooth muscle cells |
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| Institution: | 1. Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan;2. Mochida Pharmaceutical Co., Ltd., Tokyo, Japan;1. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt;2. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt;3. Department of Pharmacology, Michael Sayegh Faculty of Pharmacy, Aqaba University of Technology, Jordan;1. Department of Plastic and Hand Surgery, Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany;2. Institute of Functional and Clinical Anatomy, Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany |
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| Abstract: | The effects of elevated glucose and Eicosapentaenoic acid (EPA, 20:5) on myoinositol uptake in human aortic smooth muscle cells (HASMC) were evaluated. Myo-inositol incorporation into HASMC was dependent on an active transport system via Na+?K+ ATPase activity based on the results with Na+ deprivation and Ouabain (5 mM). Although glucose (27.5, 55 mM) inhibited 2-3H] myo-inositol uptake, the addition of EPA (3×104 M) prevented glucose-mediated inhibition. In addition, EPA potentiated Na+?K+ ATPase activity of HASMC. Since EPA decrease glucose-mediated inhibition of myo-inositol uptake, this agent might ameliorate aortic smooth muscle cell function associated with diabetes. |
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