Inhibition Kinetics of Sheep Brain Glutathione Reductase by Cadmium Ion |
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| Institution: | 1. Technische Universität Dresden, Dresden, Germany;2. University of Toronto, Canada;3. University of Montreal, Canada;1. School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui, 230009, PR China;2. School of Pharmacy, Anhui Medical University, Hefei, Anhui, 230031, PR China;1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;3. Ecological Environment Monitoring and Scientific Research Center, Changjiang River Basin Ecological Environment Administration, Ministry of Ecology and Environment, Wuhan 430014, PR China;4. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, PR China;1. Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA;2. Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR, 97239, USA |
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| Abstract: | Glutathione reductase participates in preventing lipid peroxidation by oxygen radicals which results in cellular damage. The brain is among the organs most susceptible to cadmium-induced lipid peroxidation. The mechanism of free radical generation by Cd2+ is not well understood, but it is known that Cd2+ is an inhibitor of glutathione reductase. In this study, inhibition kinetics of the brain glutathione reductase by Cd2+ was investigated. Sheep brain enzyme (11,000-fold purified) was used for this purpose. The data were analyzed by a nonlinear curve fitting program. It was found that the inhibition was competitive with respect to oxidized glutathione and uncompetitive with respect to NADPH. Inhibition constants were found as 12.3 and 9.4 μM, respectively. These findings might contribute to the understanding of the mechanism of lipid peroxidation by Cd2+ in brain. |
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