A quantitative method for analysis of radiolabelled proteoglycans synthesized by cultured human arterial smooth muscle cells |
| |
| Affiliation: | 1. Research Laboratory of Biochemical Pathology, Institute of Pathology, Aarhus Kommunehospital, University of Aarhus, DK-8000 Aarhus C Denmark [Tel. 45-8612-2233-3373; Fax 45-8612-5136];2. University Department of Clinical Chemistry, Amtsygehuset, Aarhus University Hospital, DK-8000 Aarhus C, Denmark;1. Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea;2. Department of Global Innovative Drugs, Graduate School of Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea |
| |
| Abstract: | - 1.1. Sulphate labelled proteoglycans (PG) synthesized by cultured human arterial smooth muscle cell have been quantified using an improved method based on a combination of specific enzymes and ethanol precipitation.
- 2.2. The present method gives quantitative data of PGs and subclasses allowing batchwise analysis of a large number of samples.
- 3.3. Approximately 81 % ± 1.7% (mean ± SD, n = 6) of total PGs synthesized by human arterial smooth muscle cells accumulated in medium.
- 4.4. In cell layer and medium chondroitin sulphate proteoglycan constituted 65.0% ± 0.3% and 75.8% ± 0.7% (mean ± SD, n = 3), respectively of sulphated PGs.
- 5.5. Heparan sulphate proteoglycan accounted for 26.8% ± 0.6% in cell layer and 22.6% ± 0.5% (mean ± SD, n = 3) in medium of sulphated PGs.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
