A novel angiogenesis model using interstitial cells derived from skeletal muscle |
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| Affiliation: | 1. Departments of Hygiene, Pathology I, National Defense Medical College, Tokorozawa 359, Japan;2. Departments of Biochemistry II Pathology I, National Defense Medical College, Tokorozawa 359, Japan;3. Departments of Pathology I, National Defense Medical College, Tokorozawa 359, Japan;4. Tsukuba Research Laboratories, Nippon Glaxo Ltd., Tsukuba 300-42, Japan;5. Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya 464, Japan;6. Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan;1. Business School, Jiangsu Normal University, Xuzhou 221116, China;2. Management School, China University of Mining and Technology, Xuzhou 221116, China;3. College of Economics and Management, Nanjing Forestry University, Najing 210037, China;1. Insubria University of Varese, Department of Biotechnologies and Life Sciences, Via G.B. Vico 46, I-21100 Varese, Italy;2. University of Trento, Department of Civil, Environmental and Mechanical Engineering, via Mesiano 77, I-38123 Trento, Italy;3. Department of Environmental Engineering and Physics, University of Basilicata, Viale dell’Ateneo Lucano 10, I-85100 Potenza, Italy;4. University Politehnica of Bucharest, Department of Biotechnical Systems, Faculty of Biotechnical Systems Engineering, Splaiul Independentei, nr. 313, Sector 6, 060042, Romania;5. University Lucian Blaga of Sibiu, Department of Industrial Engineering and Management, Bulevardul Victoriei, 10, 550024 Sibiu, Romania;1. Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), 3584 CT Utrecht, the Netherlands;2. University Medical Center Utrecht, Cancer Genomics Netherlands, 3584 CX Utrecht, the Netherlands;3. Max Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany |
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| Abstract: | A novel angiogenesis model using co-culture of endothelial and interstitial cells was developed, in which bovine capillary endothelial cells (BCEC) formed capillary-like structure on the monolayer of interstitial cells (like smooth muscle cells) isolated from rat skeletal muscle. The capillary formation of BCEC occurred even under serum-free conditions. Insulin stimulated the capillary growth under serum-free condition, but not BCEC growth in the single culture system. These results suggested that the insulin effect on the capillary growth was brought about indirectly through the interstitial cells, and that this co-culture system may be useful for the study of angiogenesis (especially in skeletal muscle). |
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