Effect of methyl methacrylate on mitochondrial function and structure |
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| Institution: | 1. Department of Biochemistry, Payame Noor University, Isfahan, Iran;2. Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;3. Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;4. Department of Clinical Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran;1. Centre for Biomedical Engineering (CBME), Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi 110016, India;2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (CVSc) PVNRTVU, Rajendranagar, Hyderabad 500030, Telangana, India;3. Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH Aachen University Hospital, Pauwelsstr. 30, D-52074 Aachen, Germany;4. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (CVSc) PVNRTVU, Mamnoor, Warangal 506166, Telangana, India;5. Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee 247667, Uttarakhand, India;6. Department of Pharmaceutical Chemistry, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India;7. Department of Computer Science and Engineering, University Institute of Engineering and Technology (UIET), Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India;8. Department of Mechanical Engineering, University Institute of Engineering & Technology (UIET), Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India;9. Department of Aquatic Animal Health Management, College of Fishery Science, PVNRTVU, Pebbair, Wanaparthy 509104, Telangana, India;1. Department of Environment, University of the Aegean, 81100, Mytilene, Greece;2. Department of Agricultural Development, Agrofood and Management of Natural Resources, National and Kapodistrian University of Athens, 34400, Psachna, Greece |
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| Abstract: | - 1.1. Treatment of isolated rat liver mitochondria with methyl methacrylate (MM) produced membrane disruption as evidenced by the release of citrate synthase, and changes in the ultrastructure of mitochondria.
- 2.2. At concentration 0.1%, MM uncoupled oxidative phosphorylation as evidenced by stimulation of state 4 respiration supported either by pyruvate plus malate or succinate (+rotenone) and ATP-ase activity in intact mitochondria.
- 3.3. At concentration 1% MM stimulated ATP-ase activity in intact mitochondria and succinate (+rotenone) oxidation at state 4 and was without effect on this substrate oxidation at state 3.
- 4.4. MM inhibited pyruvate plus malate oxidation either at state 3 or in the presence of uncoupling agents.
- 5.5. MM inhibited the NADH oxidase of electron transport particles at a concentration which failed to inhibit either succinic oxidase or the NADH-ferricyanide reductase activity.
- 6.6. The data presented suggest that in the isolated mitochondria MM inhibits NADH oxidation in the vicinity of the rotenone sensitive site of complex I.
- 7.7. The general conclusion is that MM may block an electron transport and to uncouple oxidative phosphorylation in rat liver mitochondria. The overall in vitro effect would be to prevent ATP synthesis which could result in cell death under in vivo conditions.
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