Use of monoclonal antibodies for identification of growth-controlling neuropeptides in the mussel Mytilus edulis (Mollusca: Bivalvia)期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Use of monoclonal antibodies for identification of growth-controlling neuropeptides in the mussel Mytilus edulis (Mollusca: Bivalvia)

Institution:	1. Equipe de recherche marine associée à IFREMER-URM 14, Laboratoire de Biologie et Biotechnologies marines, IBBA, Esplanade de la Paix, 14032 Caen Cedex, France;2. IFREMER DRIM-URM 9947, Université de Montpellier II, 2, place Eugène Bataillon CP 80, 34095 Montpellier Cedex 5, France;3. NIH/NIAID Laboratory of Malaria Research, Bdg 4, Rm B2-37, Besthesda, MD 20892, U.S.A.;1. Transplant State Center, Leon, Guanajuato, Mexico;2. Health Secretary, Guanajuato, Mexico;3. General Hospital, Celaya, Mexico;4. General Hospital, Leon, Mexico;5. General Hospital, Irapuato, Mexico;1. Burke Medical Research Institute, Circuit Repair Laboratory, 785 Mamaroneck Ave, White Plains, NY 10605, United States;2. INSERM U1106, Faculté de Médecine de la Timone, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France;1. División Ciencias de la Salud, Departamento de Ciencias Básicas, Universidad de Monterrey, San Pedro Garza García, Nuevo León, México;2. Centro de Investigaciones Biomedicas del Noreste, Monterrey, Nuevo León, México;3. Facultad de Enfermeria, Universidad Autonoma de Nuevo León, Monterrey, Nuevo León, México;4. DebRA México A.C., Guadalupe, Nuevo León, México;1. Interventional Radiology, Radiology Department, Great Ormond Street Hospital, Great Ormond Street, London, WC1N 3JH, United Kingdom;2. Specialist Neonatal and Paediatric Surgery Department, Great Ormond Street Hospital, Great Ormond Street, London, WC1N 3JH, United Kingdom;3. Bilddiagnostik, Paediatric Interventional Radiology, University Children’s Hospital, Steinwiesstrasse 75, CH-8032, Zürich, Switzerland

Abstract:	Monoclonal antibodies were developed against cerebral ganglia (CG) of the mussel Mytilus edulis by the immunization of mice with unpurified homogenates of these organs. The screening protocol of hybridoma was based upon immunohistological observations of cytocentrifugated ganglia cells. A panel of 29 monoclonal antibodies (MABs) specific of CG epitopes was harvested and subsequently used for the immunocytochemical study of CG cells. Several subpopulations of ganglia cells were specifically revealed by MABs. Identification of epitopes involved in growth control was approached via the application of a bioassay allowing the assessment of protein synthesis stimulation. MAB 42 and 46 affected amino acid incorporation induced by CG extract. These results lead to the conclusion that the epitopes recognised by these antibodies are involved in growth control. An immunoenzymatic assay was performed with CG extracts for quantitative analyses of epitopes.

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