Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5 |
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Institution: | 1. Life Science Division MS-M888, Los Alamos National Laboratory, Los Alamos, NM 87545, USA;2. Division of Radiation Hazards, National Institute of Radiological Sciences, Chiba, Japan |
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Abstract: | The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L. |
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