Acute osmotic tolerance of cultured cells of the oyster pathogen Perkinsus marinus (Apicomplexa : Perkinsida) |
| |
| Institution: | 1. School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, U.S.A.;2. Department of Zoology, University of Maryland, College Park, MD 20742, U.S.A.;1. Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey;2. Department of Food Technology, ?ebinkarahisar School of Applied Sciences, Giresun University, 28400 Giresun, Turkey;3. Department of Plant and Animal Production, Espiye Vocational School Giresun University, 28600 Giresun, Turkey;4. Department of Veterinary Medicine, Junior Technical College of Alucra Turan Bulutcu, Giresun University, 28700 Giresun, Turkey;1. CHU Rennes, Centre of Cardiothoracic and Vascular surgery, Rennes, France;2. INSERM, U1099, Rennes, France;3. University Rennes 1, Signal and Image Processing Laboratory (LTSI), Rennes, France;4. Therenva, Rennes, France;1. Laboratory of Infectious Diseases of Domestic Animals, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, PE, Brazil;2. Department of Veterinary Medicine, Federal University of Roraima, Boa Vista, RR, Brazil;3. Institute of Biological Sciences and Health (ICBS), Federal University of Alagoas, Viçosa, AL, Brazil;1. PSL Research University, ESPCI ParisTech, LSABM, 10 rue Vauquelin, 75005 Paris, France;2. Central Laboratory of Police Prefecture (LCPP), 39bis rue de Dantzig, 75015 Paris, France;3. CNRS, CBI, UMR 8231, 10 rue Vauquelin, 75005 Paris, France;4. Université Evry-Val-d’Essonne, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, F-91025 Evry, France;5. CNRS, UMR 8587, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, F-91025 Evry France;6. PSL Research University, Chimie ParisTech, 11 rue Pierre et Marie Curie, 75005 Paris, France |
| |
| Abstract: | Cultured Perkinsus marinus cells were exposed for 24 hr to salinities of 0, 3, 6, 9, 12 and 22 ppt at temperatures of 1, 5, 10, 15 and 28°C in artificial seawater (ASW) and to the same salinities at 28°C in ASW with the osmotic concentration adjusted with sucrose to the equivalent of 22 ppt. At 28°C mortality increased as salinity decreased below 22 ppt. Mortality was greater than 99% at 0 ppt and greater than 90% at 3 ppt. Mortality was 70% at 6 ppt, 43% at 9 ppt and 20% at 12 ppt. Mortality was low (<5%) and equal to that at 22 ppt in all treatments where osmotic concentration was maintained with sucrose. Mortality occurred rapidly, within 5 min of exposure to experimental conditions. In the region where mortality was most sensitive to salinity changes (6–12 ppt), lower temperature caused an increase in mortality, but the temperature effect was significant only at 9 ppt. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
