Purification and properties of the squalene-hopene cyclase from Rhodopseudomonas palustris,a purple non-sulfur bacterium producing hopanoids and tetrahymanol |
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| Affiliation: | 1. DVGW-Technologiezentrum Wasser (TZW), Karlsruher Street 84, D-76139 Karlsruhe, Germany;2. School of Environmental and Civil Engineering, Jiangnan University, Wuxi, China;3. Chinese Research Academy of Environmental Science (CRAES), Beijing, China;1. MIVEGEC, Université de Montpellier, CNRS, IRD, Montpellier, France;2. Centre of Research in Ecology and Evolution of Diseases (CREES), Montpellier, France;1. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, United States;2. Plant Resilience Institute, Michigan State University, East Lansing, MI, United States |
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| Abstract: | The squalene-hopene cyclase of the hopanoid- and tetrahymanol-producing Rhodopseudomonas palustris was released from the isolated membranes by CHAPS and purified to homogeneity by succesive chromatography on DEAE Sephacel, Octyl Sepharose, and Blue Sepharose. The enzyme has a molecular weight of 70 kDa as determined by SDS-PAGE and an isoelectric point at about pH 5.O. The enzyme activity has a maximum at 30°C and at pH 6.5. No production of tetrahymanol could be demonstrated by using either crude or purified cyclase preparations. |
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