Primary Spermatocyte-Specific Cre Recombinase Activity in Transgenic Mice |
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Authors: | Chung Sanny S W Cuzin François Rassoulzadegan Minoo Wolgemuth Debra J |
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Institution: | Department of Genetics and Development, Columbia University Medical Center, 630 W. 168th Street, New York, NY 10032, USA. |
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Abstract: | We have evaluated the specificity of Cre recombinase activity in transgenic mice expressing Cre under the control of the synatonemal complex protein 1 (Sycp1) gene promoter. Sycp1Cre mice were crossed with the ROSA26 reporter line R26R, to monitor the male germ cell stage-specificity of Cre activity as well as to verify that Cre was not active previously during development of other tissues. X-gal staining detected Cre-mediated recombination only in testis. Detailed histological examination indicated that weak Cre-mediated recombination occurred as early as in zygotene spermatocytes at stage XI of the cycle of the seminiferous epithelium. Robust expression of X-gal was detected in early to mid-late spermatocytes at stages V-VIII. We conclude that this transgenic line is a powerful tool for deleting genes of interest specifically during male meiosis. |
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Keywords: | Cre recombinase Sycp1Cre ROSA26 primary spermatocytes |
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