A repetitive probe for FISH analysis of bovine interphase nuclei |
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| Authors: | Wafa Slimane Daniel Vaiman Sophie Godard Anne Vaiman Edmond Cribiu Jean-Paul Renard |
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| Institution: | 1.Unité biologie du développement, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France;2.Services techniques de l''Union national des coopératives d''élevage et d''insémination artificielle, 13, rue Jouët, BP 65, 94703 Maisons-Alfort, France;3.Génétique biochimique et cytogénétique, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex France |
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| Abstract: | The purpose of this study was to generate repetitive DNA sequence probes for the analysis of interphase nuclei by fluorescent in situ hybridisation (FISH). Such probes are useful for the diagnosis of chromosomal abnormalities in bovine preimplanted embryos. Of the seven probes (E1A, E4A, Ba, H1A, W18, W22, W5) that were generated and partially sequenced, five corresponded to previously described Bos taurus repetitive DNA (E1A, E4A, Ba, W18, W5), one probe (W22) shared no homology with other DNA sequences and one (H1A) displayed a significant homology with Rattus norvegicus mRNA for secretin receptor transmembrane domain 3. Fluorescent in situ hybridisation was performed on metaphase bovine fibroblast cells and showed that five of the seven probes hybridised most centromeres (E1A, E4A, Ba, W18, W22), one labelled the arms of all chromosomes (W5) and the H1A probe was specific to three chromosomes (ch14, ch20, and ch25). Moreover, FISH with H1A resulted in interpretable signals on interphase nuclei in 88% of the cases, while the other probes yielded only dispersed overlapping signals. |
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| Keywords: | satellite DNA FISH bovine centromere |
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