Enzymatic reactions by five chalcone synthase homologs from hop (Humulus lupulus L.) |
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Authors: | Okada Yukio Sano Yukie Kaneko Takafumi Abe Ikuro Noguchi Hiroshi Ito Kazutoshi |
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Affiliation: | Bioresources Research and Development Laboratories, Sapporo Breweries, Ltd, Gunma, Japan. yukio.okada@sapporobeer.co.jp |
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Abstract: | The enzyme activities encoded in five cDNAs for chalcone synthase (CHS) homologs from hop were investigated. Only valerophenone synthase (VPS) and CHS_H1 showed both naringenin-chalcone and phlorisovalerophenone forming activity. Narigenin-chalcone production by VPS was much lower than by CHS_H1. Therefore, it is highly possible that flavonoid depends mainly on CHS_H1, while bitter acid biosynthesis depends mainly on VPS and CHS_H1. |
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