A comparison of barley isolated microspore and anther culture and the influence of cell culture density |
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Authors: | P A Davies S Morton |
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Institution: | (1) SARDI, Field Crops Pathology Unit, GPO Box 397, Adelaide SA 5001, Australia Fax-No.: +61-8-8303-7321 E-mail: davies.phil@pi.sa.gov.au, AU |
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Abstract: | Comparisons were made between the efficiency of barley plant regeneration from anther culture (AC) and isolated microspore
culture (IMC) for the European winter cultivar `Igri' and the spring F1 Australian breeder's hybrid Amagi Nijo×WI2585. In both cases, IMC produced a higher number of green regenerant plantlets
per anther than AC. For `Igri' there was a 100- to 200-fold improvement and for Amagi Nijo×WI2585 there was a five- to ninefold
improvement of IMC over AC. To improve the consistency and reliability of the IMC method, we investigated several parameters,
including maltose concentration, subculture protocol, microspore plating density and colony plating density. Subculturing
during the liquid culture phase produced no significant improvement in the number of microspores developing into colonies.
The optimal concentration of maltose in the liquid induction medium was found to be 90 g l–1. Both microspore plating density and colony plating density were found to influence plant regeneration. Microspores produced
the highest numbers of colonies when plated at densities greater than 5×104 ml–1, and colonies produced optimal numbers of green plantlets when plated at 12.5–25 colonies/cm2.
Received: 23 March 1997 / Revision received: 29 May 1997 / Accepted: 25 June 1997 |
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Keywords: | Microspore Anther Doubled haploid Barley Hordeum vulgare |
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