The use of histology in the study of plant tissue culture systems—Some practical comments |
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Authors: | Edward C Yeung |
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Institution: | (1) Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alberta, Canada |
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Abstract: | Summary Histological methods have contributed significantly to our understanding of in vitro culture systems. A good histological
study based on anatomical and histochemical changes provides insight into cellular processes and provides clues that allow
for the proposal of hypotheses for further experimentation. This article serves to draw attention to the use of a histological
approach to one’s experimental system. Some of the common mistakes in the handling and processing of explants are discussed.
A protocol for the plastic embedding method is detailed. |
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Keywords: | histology paraffin and plastic embedding methods photomicrography staining |
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