Turgor regulation and cytoplasmic free Ca2+ in the algaLamprothamnium |
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Authors: | Yoshiji Okazaki Yasuaki Yoshimoto Yukio Hiramoto Masashi Tazawa |
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Affiliation: | (1) Department of Biology, Faculty of Science, University of Tokyo, Hongo 7-3-1, 113 Tokyo, Japan;(2) Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444 Aichi, Japan;(3) Biological Laboratory, Tokyo Institute of Technology, 152 Tokyo, Japan;(4) Present address: Department of Biology, Osaka Medical College, Sawaragi-cho 2-41, 569 Osaka, Japan;(5) Present address: The University of the Air, Wakaba 2-11, 260 Chiba, Japan |
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Abstract: | Summary In internodal cells ofLamprothamnium succinctum, turgor regulation in response to hypotonie treatment is inhibited by lowering external Ca2+ concentration ([Ca2+]e) from 3.9 (normal) to 0.01 (low) mM. In order to clarify whether a change in the cytoplasmic free Ca2+ concentration ([Ca2+]c) is involved in turgor regulation, the Ca2+ sensitive protein aequorin was injected into the cytoplasm of internodal cells. A large transient light emission was observed upon hypotonic treatment under normal [Ca2+]e but not under low [Ca2+]e. Thus hypotonic treatment induces a transient increase in [Ca2+]c under normal [Ca2+]e but not under low [Ca2+]e.Abbreviations ASW artificial sea water - i cellular osmotic pressure - [Ca2+]c cytoplasmic free Ca2+ concentration - EDTA ethylenediamine-tetraacetic acid - EGTA ethylenglycol-bis(-aminoethyl ether(N,N-tetraacetic acid - [Ca2+]e external Ca2+ concentration - e external osmotic pressure - GM glass micropipette - GP glass plate - HEPES N-2-hydroxyethylpiperazine-N-2-ethansulfonic acid - MS microscope stage - OL objective lens - PIPES piperazine-N-N-bis(2-ethanesulfonic acid) - W Weight |
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Keywords: | Aequorin Calcium Characeae Lamprothamnium Turgor regulation |
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