Identification of 10 882 porcine microsatellite sequences and virtual mapping of 4528 of these sequences |
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Authors: | Karlskov-Mortensen P Hu Z L Gorodkin J Reecy J M Fredholm M |
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Institution: | Department of Animal and Veterinary Basic Sciences, Division of Genetics, Faculty of Life Sciences, University of Copenhagen, Denmark. pkm@life.ku.dk |
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Abstract: | A total of 10 882 porcine microsatellite repeats were identified in genomic shotgun sequences from the Sino-Danish Pig Genome Sequencing Consortium (http://www.piggenome.dk). Of these, 4528 microsatellites were placed on a pig-human comparative map by blast analysis of porcine sequences against the human genome (blast cut-off threshold =1 x 10(-5)). All microsatellite sequences placed on the comparative map are accessible at http://www.animalgenome.org/QTLdb/pig.html. These sequences increase the number of identified microsatellites in the porcine genome by several orders of magnitude. They are a new resource of microsatellite sequences for generating markers to be used in linkage studies and in fine mapping and positional cloning of quantitative trait loci. |
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Keywords: | comparative map genetic marker linkage map microsatellite pig positional quantitative trait loci mining |
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