Stable carbon isotope discrimination in the smut fungusUstilago violacea |
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| Affiliation: | 1. Centre GEOPAC, Laboratoire de Geobiodiversite et Patrimoine Naturel, Université Mohammed V de, Institut Scientifique Rabat, Maroc;2. University Mohammed VI Polytechnic, Agrobiosciences Program, Lot 660, Hay Moulay Rachid, Benguerir, Morocco;3. Environment and Health Team, Polydisciplinary Faculty of Safi, Cadi Ayyad University, Safi, Morocco;4. Medical Biotechnology Laboratory (MedBiotech), Rabat Medical & Pharmacy School, Mohammed V University in Rabat, 6203 Rabat, Morocco;5. Department of Biology, Faculty of Science, University Sidi Mohamed Ben Abdellah, Fez, Morocco;6. Mohammed V University in Rabat, LPCMIO, Materials Science Center (MSC), Ecole Normale Supérieure, Rabat, Morocco;7. Laboratory of Histology, Embryology, and Cytogenetic, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco;8. Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, 11566, Egypt;9. Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, 11835, Egypt;10. Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya, Turkey;11. Laboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, and Genomic Center of Human Pathologies, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco;1. Dpto. Biología Animal, Biología Vegetal y Ecología. Universidad de Jaén. Campus las Lagunillas s/n, E-23071, Jaén, Spain;2. Dpto. Ciencias Ambientales. Universidad de Castilla–La Mancha. Campus Fábrica de Armas, E-45071, Toledo, Spain |
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| Abstract: | Haploid strains 15.10, 1.C429, and 1.C2y and diploid strain JK2 ofUstilago violacea were grown on one or more of the following carbon sources: glucose, sucrose, maltose, inulin, starch, inositol, glycerol, casein, and yeast extract. The media, both before and after fungal growth, and the fungal cells were analyzed for 13C/12C content (δ13C values) using an isotope ratio mass spectrometer after combustion to CO2. In all cases, the used and unused media had identical δ13C values. Strain 15.10 had significantly less13C than the media when grown on glucose, sucrose, maltose, and inositol; significantly more13C when grown on inulin, starch, and glycerol; and no significant difference in δ13C values when grown on casein and yeast extract media. Other haploid strains responded similarly to 15.10. Diploid strain JK2 was also depleted in13C when grown on glucose and enriched in13C when grown on glycerol; however, JK2 was slightly depleted in13C when grown on casein, whereas all the tested haploid strains were enriched in13C. |
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