Copper uptake inAspergillus niger during batch growth and in nongrowing mycelial suspensions |
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| Affiliation: | 1. K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea;2. Department of Life Systems, Sookmyung Women''s University, Cheongpa-ro 47-gil 100, Yongsan-gu, Seoul 04310, Republic of Korea;3. Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do 220-702, Republic of Korea;1. University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia;2. PerkinElmer Chemagen Technologie GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler, Germany;3. Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA;4. Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany;5. Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany;6. Institute of Chemistry Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia;7. Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA;1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, PR China;2. State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China;1. Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, United States;2. Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang 310058, PR China |
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| Abstract: | Copper accumulation by the filamentous fungusAspergillus niger from a glucose mineral salts medium containing copper in the concentration range 16 to 157 μM was maximal in the lag phase of growth. In the subsequent linear growth phase, the mycelial copper contents were dramatically reduced on a per gram dry weight basis. The fungal mycelium exhibited pelleted morphology and exponential growth was not apparent. The medium pH was reduced during growth in flask cultures, but this was not responsible for the reduction in copper uptake as indicated by the similar effect in cultures grown in a stirred-tank fermenter with electronic maintenance of pH at 5.5. Voltammetric analysis of medium which had supported growth of the fungus showed that copper added at a final concentration of 40 μM was complexed. Energy-dependent copper uptake from 2-(N-morpholino)ethanesulfonic acid buffer at pH 5.5 containing 40 μM copper could not be demonstrated in nongrowing mycelium. Incubation at 4°C reduced copper uptake while the presence of 10 mM glucose or preincubation of the mycelium in 1 mM sodium azide had no effect on copper uptake. |
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