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Prolyl endopeptidase inhibitory activity of chitosan sulfates with different degree of deacetylation |
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| Authors: | Jae-Young Je Pyo-Jam Park Se-Kwon Kim |
| Institution: | aDepartment of Chemistry, Pukyong National University, 599-1, Daeyon 3-dong, Nam-Gu, Busan 608-737, South Korea bDepartment of Biotechnology, Konkuk University, Chungju 380-701, South Korea |
| Abstract: | Three kinds of partially deacetylated chitosan, 90% deacetylated chitosan, 75% deacetylated chitosan and 50% deacetylated chitosan, were prepared from crab chitin by N-deacetylation with 40% (w/w) sodium hydroxide solution for different durations. In order to improve biological activity and solubility, their sulfated derivatives were prepared, and prolyl endopeptidase (PEP) inhibitory activities were investigated. Fifty percent-deacetylated chitosan sulfate (50-CS) exhibited the highest inhibitory activity, and inhibition rate was a dose-dependant. In addition, Dixon plots suggested that 50-CS was act as competitive inhibitor, and the inhibition constant (Ki) was 2.6 mg/ml. |
| Keywords: | Hetero-chitosans Sulfated derivatives Prolyl endopeptidase Competitive |
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