Cytochemical localization of alkaline phosphatase in the cell membrane ofDictyostelium discoideum amebae |
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| Authors: | I. Glomp, D. Schä fer B. Hess |
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| Affiliation: | (1) Max-Planck-Institut für Ernährungsphysiologie, Rheinlanddamm 201, D-4600 Dortmund, Germany;(2) Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, D-4600 Dortmund, Germany |
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| Abstract: | Summary We demonstrated that alkaline phosphatase was localized on the cell membrane ofDictyostelium discoideum amebae and on isolated plasma membranes. The enzyme activity was specifically inhibited by 0.01 M KCN or cysteine. The same method could also be applied to baker's yeast and MDCK cells (dog kidney cells in vitro). |
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