家兔迟发性面瘫模型建立及药物干预研究

目的:优化迟发性面瘫的建模方法,并对药物的神经保护作用进行观察。方法:家兔48只共96侧面神经,分A、B、C、D 4个实验组,以一侧面神经进行实验处理,另一侧为自身对照。A组:直视下钳夹损伤桥池段面神经;B组:直视下向桥小脑角注射动脉血,以诱导血管痉挛;C组:处理因素=A组+B组;D组:在C组基础上,应用药物(强的松+丹参+维生素B1+维生素B12)干预。观察家兔面瘫,并做面神经病理切片,比较各组间迟发性面瘫发生率、面瘫持续时间及预后。结果:面瘫发生情况:A组6只家兔(6/11,54.5%)出现迟发性面瘫;平均面瘫持续时间为13.2天。B组有2只(2/12,16.7%)出现迟发性面瘫,平均持续8天。C组6只(6/12,50%)家兔出现迟发性面瘫,平均持续14.3天。D组4只(4/12,33.33%)出现迟发性面瘫,平均持续6天。所有自身对照侧均无面瘫发生。病理:各组均见神经纤维水肿;A、C两组呈高度水肿改变,神经束周围结构紊乱;B组见神经内血管细小,而水肿较A、C两组轻微;D组呈轻度水肿改变。结论:C组出现迟发性面瘫几率高,是较好的模型;联合应用强的松、丹参、维生素B1、维生素B12虽不能防止迟发性面瘫发生,但可使迟发性面瘫病程明显缩短。

Rabbit Model of Delayed Facial Paralysis and Drug Intervention

ABSTRACTObjective:Optimization ofmodeling of delayed facial paralysis.The neuroprotective effect of several drugswere observed.Methods: Forty-eight rabbits were assigned to A, B, C, D 4 experimental groups, with one side of the facial nerve to the experimentaltreatment, and the other side of self-control. A: The cisternal section of the facial nerve was injured by clamp. B: Injection of arterialblood to the cerebellopontine angle to induce vasospasm. C: treatment factor = group A + group B. D group: C group plus the applicationof drug (prednisone + SM + vitamin B1 + vitamin B12) intervention. The rabbit facial paralysis was observed. And facial nerve biopsywas done. The incidence of delayed facial paralysis, duration and prognosis were compared. Results: The incidence of facial paralysis: ingroup A 6 rabbits (6 / 11, 54.5%) had delayed facial paralysis; the average duration of facial paralysis was 13.2 days. In group B, 2 (2 /12, 16.7%) had delayed facial paralysis, the average duration was 8 days. In group C 6 (6 / 12, 50%) had delayed facial paralysis, and theaverage duration was 14.3 days. In group D 4 (4 / 12, 33.33%) had delayed facial paralysis, the average duration was 6 days. There wasno facial paralysis in all self-control sides. Pathology: Nerve fiber edema was seen in each group ; groups A, C showed a high degree ofedema, with nerve bundles around the structural disorder; group B showed small blood vessels within the nerve, and mild edema comparedwith groups A, C; group D showed a slight edema change. Conclusion: Group C had higher risk of delayed facial paralysis, so it isa better model. Combination of prednisone, Salvia, vitamin B1, vitamin B12, although not able to prevent the occurrence of delayed facialparalysis, but the duration of the delayed facial paralysis was significantly shortened.