|
|||||
|
|
| 骨髓间充质干细胞移植对VCD 所致卵巢损伤的修复研究 | |
| 引用本文： | 王琰,杨瑛,刘兵,陈宥艺,陈冬波.骨髓间充质干细胞移植对VCD 所致卵巢损伤的修复研究[J].现代生物医学进展,2011,11(10):1844-1850. |
| 作者姓名： | 王琰 杨瑛 刘兵 陈宥艺 陈冬波 |
| 作者单位： | 1. 第四军医大学唐都医院妇产科,陕西,西安,710038 2. 解放军307医院妇产科,北京,100071 3. 解放军307医院肿瘤学研究室,北京,100071 |
| 基金项目： | 国家"863"计划资助子课题 |
| 摘 要： | 目的:探讨小鼠骨髓间充质干细胞(MSCs)移植对去氧乙烯基环己烯(VCD)所致卵巢早衰治疗的可行性。方法:采用VCD(160mg kg-1,day-1)连续腹腔注射来诱导小鼠卵巢早衰。每侧卵巢注射转染了绿色荧光基因小鼠骨髓来源的MSCs,于移植后14、28天及45天,取各组血液标本及卵巢组织,同时观察小鼠动情周期的变化;酶联免疫法检测血清FSH、LH水平,显微镜下观察MSC在卵巢的分布。结果:MSCs移植后各组均可见绿色荧光,并且主要分布于卵巢间质区,卵巢泡膜细胞区也可见绿色荧光细胞。MSCs组动情周期较实验对照组缩短,FSH与LH水平较实验对照组低,差异具有显著性。结论:骨髓间充质干细胞可改善卵巢早衰小鼠的卵巢内分泌功能,并且长时间存在于卵巢组织。骨髓间充质干细胞可能成为卵巢早衰治疗的新方法。 |
| 关 键 词： | 骨髓间充质干细胞 VCD 卵巢早衰 模型 小鼠 |
Bone Marrow Mesenchymal Stem Cell Transplantation for Premature Ovarian Failure Induced by 4-vinylcyclohexene Diepoxide |
|
| WANG Yan,YANG Ying,LIU Bing,CHEN You-yi,CHEN Dong-bo.Bone Marrow Mesenchymal Stem Cell Transplantation for Premature Ovarian Failure Induced by 4-vinylcyclohexene Diepoxide[J].Progress in Modern Biomedicine,2011,11(10):1844-1850. | |
| Authors: | WANG Yan YANG Ying LIU Bing CHEN You-yi CHEN Dong-bo |
| Institution: | 1 Department of obstetrics and gynecology,tangdu Hospital,Forth Military Medical University,Xi’an 710038,China;2 Department of obstertrics and Gynecology,307 Hospital of PLA,Beijing 10071,China;3 Laboratory of oncology,307Hospital of PLA,Beijing 10071,China) |
| Abstract: | Objective:To investigate the therapeutic potency of MSCs transplantation on premature ovarian failure induced by 4-vinylcyclohexene diepoxide(VCD).Methods:Female C57 mice were dosed with VCD(160mg kg-1 day-1,ip) daily to induce ovarian failure.GFP-labeled BMSCs were injected into the ovary.The FSH and LH in the serum were detected and the ovaries were collected at 14d,28d and 45d after transpantatipn.GFP-labeled MSCs were tracked in the ovaries.Ovarian follicle populations were also determined.Results:There... |
| Keywords: | VCD |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《现代生物医学进展》浏览原始摘要信息 | |
| 点击此处可从《现代生物医学进展》下载免费的PDF全文 | |