系统解剖学立体化双语教学资源建设的探讨

系统解剖学是医学生最为重要的专业基础课程之一。近年来,我们一直坚持双语教学,2008年我校系统解剖学获得首批国家双语教学示范课程。为建设好双语教学示范课程,我们积极开展解剖学双语教学探索和实践,着重于该门课程的立体化双语教学资源的建设,以纸质教材为主体,利用不同教学媒体的优点来呈现解剖学不同的教学内容,形成一个立体化的双语教学资源,并应用于教学实践活动中,达到提高学生专业英语理解能力,灵活运用外语思维解决医学问题能力的目标。

Construction of Three-dimensional Bilingual Teaching Resourcesin Systematic Anatomy

Objective: To study a convenient and effective technique for heat induced antigen retrieval in BrdU immunohistochemicalstaining. Methods: We explored three heat induced antigen retrieval methods in this experiments: 1) Boiled floating sectionsmethod; 2) Boiled patches method; 3) Improved patches method. Put the glass slides posted with frozen sections into a kind ofthermostatic and closed device we designed for antigen retrieval. Then we did the BrdU immunohistochemical staining. Pictures werecollected to compare the three heat induced antigen retrieval methods. And we did the BrdU immunofluorescent co-labelling with NeuN,p-CERB, DCX and collect pictures to find out whether the improved patches method could be used in BrdU immunofluorescentalstaining. Results: 1) Boiled floating sections method. The frozen sections were too shrunken to be used in the following steps afterretrieved the antigen in this way. 2) Boiled patches method. Several BrdU labeled neural stem cells could be confirmed, but thebackground was too dark. And some of the patches blistered, then a great deal of false positive cells were found in the blistered areas. 3)Improved patches method. The positive products of BrdU immunohistochemistry and immunofluorescent co-labeling with NeuN, p-CERB,DCX were distinct and the background was controlled well. Conclusion: Our antigen retrieval technique could fully expose the BrdUantigen , the DAB staining and immunohistochemical staining was controlled well. Therefore, it is an improved technique of antigenretrieval in BrdU immunohistochemical staining.