| 杨梅黄酮减弱MPP+ 诱导的MES23.5 细胞的细胞毒性 |
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| 引用本文： | 张凯马泽刚王俊谢安木. 杨梅黄酮减弱MPP+ 诱导的MES23.5 细胞的细胞毒性[J]. 现代生物医学进展, 2011, 11(10): 1830-1833 |
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| 作者姓名： | 张凯马泽刚王俊谢安木 |
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| 作者单位： | 国家生理学重点,培育,学科,山东省神经相关疾病的机制与防治重点实验室,青岛大学医学院生理学教研室,青岛大学医学院附属医院神经内科,山东,青岛,266071 |
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| 基金项目： | 国家自然科学基金项目，青岛市科技局项目 |
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| 摘 要： | 目的:探讨杨梅黄酮对MPP+处理的MES23.5细胞的保护作用。方法:实验分为空白对照组,MPP+处理组,MPP+和杨梅黄酮共处理组,分别处理MES23.5细胞24h后,应用Hoechst 33258染色观察各组细胞细胞核形态的改变,应用RT-PCR技术观察Bcl-2和Bax mRNA表达的改变。结果:杨梅黄酮能明显抑制MPP+引起的MES23.5细胞核固缩,核碎裂等形态学的改变,杨梅黄酮还可以对抗MPP+处理造成的MES23.5细胞的Bcl-2/Bax比率的降低。结论:杨梅黄酮对MPP+处理的MES23.5细胞具有保护作用。
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| 关 键 词： | 杨梅黄酮 MPP+ 凋亡 MES23.5 cells |
Myricetin Attenuates MPP+-induced Cytotoxicity in MES23.5 Cells |
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| ZHANG Kai,MA Ze-gang,WANG Jun,XIE An-mu. Myricetin Attenuates MPP+-induced Cytotoxicity in MES23.5 Cells[J]. Progress in Modern Biomedicine, 2011, 11(10): 1830-1833 |
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| Authors: | ZHANG Kai MA Ze-gang WANG Jun XIE An-mu |
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| Affiliation: | ZHANG Kai,MA Ze-gang,WANG Jun,XIE An-mu(Department of Physiology,Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines:Physiology,Medical College of Qingdao University,Department of Neurology,the affiliated hospital of Medical College of Qingdao University.Qingdao,266071,China) |
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| Abstract: | Objective: To investigate the neuroprotective effect of myricetin on 1-methyl-4-phenylpvridinium (MPP+)-treated MES23.5 cells. Methods: MES23.5 cells were treated with vehicle, MPF, MPF and myricetin for 24h respectively. By Hoechst staining and RT-PCR, the nuclear morphological changes and the Bcl-2 and Bax mRNA levels were investigated. Results: The results showed mat myricetin treatment significantly attenuate MPF-induced cell apoptosis. MPF treatment induced decrease of Bcl-2/Bax mRNA ratio was also inhibited by myricetin. Conclusion: Myricetin may protect against MPP+-induced cytotoxicity in MES23.5 cells. |
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| Keywords: | MPP+ MES23.5 cells |
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