The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio) |
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Authors: | Seki Shinsuke Kouya Toshimitsu Valdez Delgado M Jin Bo Hara Takao Saida Naoya Kasai Magosaburo Edashige Keisuke |
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Institution: | Laboratory of Animal Science, College of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan. |
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Abstract: | To identify a stage feasible for the cryopreservation of zebrafish oocytes, we investigated the permeability to water and cryoprotectants of immature (stage III) and mature (stage V) oocytes. The permeability to water (microm/min/atm) of immature oocytes at 25 degrees C (0.37) was significantly higher than that of mature oocytes (0.10). The permeability (x10(-3)cm/min) of immature oocytes to ethylene glycol, propylene glycol, and Me(2)SO (1.49-3.03) at 25 degrees C was substantially higher than that of mature oocytes approximately 0. The permeability of immature oocytes to glycerol was also high (1.75), although the permeability could not be measured in mature oocytes. Immature oocytes would be more suitable than mature oocytes for conservation of the zebrafish. |
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Keywords: | Zebrafish Oocyte Permeability Water Cryoprotectant |
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